Líneas de Investigación
El objetivo de nuestro trabajo es comprender los mecanismos moleculares involucrados en la remodelación dela pared vegetal. Para ello he centrado mi trabajo en el desarrollo y aplicaciones de técnicas de genómica funcional. Así, el principal objetivo de trabajo es entender la dinámica de la pared celular en dos aspectos: formación y desensamblaje de la pared vegetal. Se aplican en el laboratorio, técnicas tales como: RAPD, microsatélites, AFLP, RNA-seq. El estudio de transcritos, clonamiento, expresiónheteróloga y caracterización ha sido abordado en el laboratorio, en este sentido técnicas de genómica funcional, generación de bases de dato genómica y bioinformáticaestructural han sido implementadas.
Salazar, R., Pollmann, S., Morales-Quintana, L., Herrera, R., Caparrós-Ruiz, D., Ramos P. 2018. In seedlings of Pinus radiata, jasmonic acid and auxin are differentially distributed on opposite sides of tilded stems affecting lignin monomer biosynthesis and composition. Plant Phisiology and Biochemistry 135: 215-223.
Aceituno-Valenzuela, U., Covarrubias, M.P., Aguayo, M.F., Valenzuela-Riffo, F., Espinoz, A., Gaete-Eastman, C., Herrera, R., Handford, M., Norambuen, L. 2018. Identification of a type II cystatin in Fragaria chiloensis: A proteinase inhibitor differentially regulated during achene development and in response to biotic stress-related stimuli. Plant Physiology and Biochemistry 129: 158-167
Carrasco-Orellana, C., Stappung, Y., Mendez-Yañez, A., Allan, A.C, Espley, R.V., Plunkett, B.J., Moya-Leon, M.A., Herrera, R. 2018. Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit. Scientific Report. 10.1038/s41598-018-28226-y
Gomez, R., Gonzalez, J., Herrera, R., Ramos, P., 2018. MYB Transcription Factors and a Putative Flavonoid Transporter ABCC-Like are Differentially Expressed in Radiata Pine Seedlings Exposed to Inclination. J. Plant Growth Regul. 37, 64–75. doi:10.1007/s00344-017-9707-5
Herrera R. (2017) Transcription analysis of softening-related genes during postharvest of papaya fruit (Carica papaya L. Pococí hybrid). Postharverst biology and technology 125:42-51
Morales-Quintana, L., Faundez, C., Herrera, R., Zavaleta, V., Ravanal, M.C., Eyzaguirre, J., Moya-Leon, M.A. (2017) Biochemical and structural characterization of Penicillium purpurogenum alpha-D galactosidase: Binding of galactose to an alternative pocket may explain enzyme inhibition. Carbohydrate Research 448, 57-66
Morales L , Herrera R, Ramos P. (2017) Transcriptional and computational study of expansins differentially expressed in response to inclination in radiata pine. Plant Physiology and Biochemistry 115:12-24.
Méndez-Yañez, Á., Beltrán, D., Campano-Romero, C., Molinett, S., Herrera, R., Moya-León, M.A., Morales-Quintana, L., 2017. Glycosylation is important for FcXTH1 activity as judged by its structural and biochemical characterization. Plant Physiol. Biochem. 119: 200–210.
Opazo, M.C., Lizana, R., Stappung, Y., Davis, T.M., Herrera, R., Moya-León, M.A. 2017. XTHs from Fragaria vesca: Genomic structure and transcriptomic analysis in ripening fruit and other tissues. BMC Genomics 18. doi:10.1186/s12864-017-4255-8
Zerpa-Catanho, D., Esquivel, P., Mora-Newcomer, E., Sáenz, M. V, Herrera, R., Jiménez, V.M., 2017. Transcription analysis of softening-related genes during postharvest of papaya fruit (Carica papaya L. “Pococí” hybrid). Postharvest Biol. Technol. 125, 42–51
Espinoza, A., Contreras, R., Zuniga, G., Herrera, R., Moya-Leon, M.A., Norambuena, L., Handford. M. 2016. FcLDP1, a Gene Encoding a Late Embryogenesis Abundant (LEA) Domain Protein, Responds to Brassinosteroids and Abscisic Acid during the Development of Fruits in Fragaria chiloensis. Frontiers in Plant Science.
Gaete-Eastman C., Morales-Quintana L., Herrera R., Moya-León MA. (2015). In silico analysis of the structure and binding site features of expansin protein from mountain papaya fruit (VpEXP2), through molecular modelling, docking and dynamics simulation studies. Journal of Molecular Modelling.21:115 doi: 10.1007/s00894-015-2656-7.
Bassil NV., Davis TM.,AlperinE., Amaya I., Bellon F., Brew F., Denoyes B., Dijk T., Koehorst-van Putten H., Ficklin S., Iezzoni A., Mahoney L., Main D., Mittman M., Monfort A., Peace C., Pirani A., Rosyara U., Sargent DJ., Bianco L, Webster T., Wood D., Yang Y., Zhang H., Whitaker VM., Verma S., Herrera R., van de Weg E. 2015. Development and Preliminary Evaluation of a 90K Axiom SNP Array in the Allo-octoploid Cultivated Strawberry Fragaria ×ananassa.BMC Genomics 16:155. doi:10.1186/s12864-015-1310-1
Salvatierra A., Pimentel P., Moya-León MA., Herrera R. (2014). Biosynthesis of flavonoids in achenes of Fragaria chiloensisspp. chiloensis. Blacpma. 13(4):406-414.
Valenzuela C., Ramos P., Carrasco C., Moya-Leon Ma., Herrera R. (2014). Cloning and characterization of a xyloglucan endo-transglycosylase/hydrolase gene expressed in response to inclination in radiata pine seedlings.Tree Genetics and Genomes. 10:1305-1315. doi: 10.1007/s11295-014-0762-9
Garces M., Le Provost G., Lalanne C., Claverol S., Barre A., Plomion C., Herrera R. (2014). Proteomic analysis during ontogenesis of secondary xylem in maritime pine.Tree Physiol. 34:1263-1277. doi:10.1093/treephys/tpt117.
Morales-Quintana L., Nunez-Tobar M., Moya-Leon MA., Herrera R. (2013).Molecular Dynamics Simulation and Site-Directed Mutagenesis of Alcohol Acyltransferase: A Proposed Mechanism of Catalysis. Journal of Chemical Information and Modeling53: 2689-2700 doi: 10.1021/ci400409s
Salvatierra, A., Pimentel, P., Moya-Leon, M.A., Herrera, R. (2013) Increased accumulation of anthocyanins in Fragariachiloensis fruits by transient suppression of FcMYB1 gene.Phytochemistry 90: 25-36. (doi:10.1016/j.phytochem.2013.02.016)
Ramos P., Herrera R. (2013) Anatomical changes of xylem cells in stem of Pinusradiata seedlings exposed to inclination and ethylene. BiologiaPlantarum, 57: 525-530.
Opazo, M.C., Lizana, R., Pimentel, P., Herrera, R., Moya-León, M.A. (2013) Changes in the mRNA abundance of FcXTH1 and FcXTH2 promoted byhormonal treatments of Fragariachiloensisfruit. Postharvest Biology and Technology, 77: 28-34.(doi:10.1016/j.postharvbio.2012.11.007)
González, G., Fuentes, L., Moya-León, M.A., Sandoval, C., Herrera, R. (2013) Characterization of two PR genes from Fragariachiloensis in response to Botrytis cinerea infection: A comparison with Fragaria x ananassa. Physiological and Molecular Plant Pathology 82: 73-80. (doi:10.1016/j.pmpp.2013.02.001)
Galaz, S., Morales-Quintana, L., Moya-Leon, M.A., Herrera, R. (2013) Structural analysis of the alcohol acyltransferase protein family from Cucumismelo shows that enzyme activity depends on an essential solvent channel. FEBS Journal 280: 1344-1357. (doi:10.1111/febs.12127)
2008 – 2010 Respuesta gravitrópica y diversidad genética en coníferas Ecos-Conicyt C07B01
2012 Proyecto MEC 80122010. “Genomic Studies and Molecular Marker Development in the Chilean Strawberry”
2012-2014 Early response to inclination in young seedlings of radiata pine: characterizing key molecular players. Fondecyt-Regular Nº1120635
2012-2015 Investigadorresponsable Proyecto Anillo (ACT 1110) “Advanced center for the integrative study of fruit ripening: Molecular and physiological basis of complex quality traits of the Chilean strawberry fruit (ACIS)”.
2013-2016 Marker-trait associations, population structure and genetic evaluation of Eucalyptus cladocalyx under arid environmental conditions (Co-investigador).
2013 Red en biotecnología agrícola: vinculación académica UTALCA-Michigan State University. Relaciones Internacionales Conicyt. REDES120029. Responsable.
2014-2020 Proyecto inserción en la academia (PAI-Conicyt)
2015-2019 Understanding the involvement of MADS-box transcription factors from radiata pine inwood forming tissue: unraveling the molecular mechanisms in cell wall dynamicsduring wood formation. Fondecyt Regular Nº1150964